LOCUS       sequence-153901-         769 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TFF2_pCSdest
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(527..551)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    595..729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(619..638)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     673..692
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcac aagtttgtac
      121 aaaaaagcag gcaccatggg acggcgagac gcccagctcc tggcagcgct cctcgtcctg
      181 gggctatgtg ccctggcggg gagtgagaaa ccctccccct gccagtgctc caggctgagc
      241 ccccataaca ggacgaactg cggcttccct ggaatcacca gtgaccagtg ttttgacaat
      301 ggatgctgtt tcgactccag tgtcactggg gtcccctggt gtttccaccc cctcccaaag
      361 caagagtcgg atcagtgcgt catggaggtc tcagaccgaa gaaactgtgg ctacccgggc
      421 atcagccccg aggaatgcgc ctctcggaag tgctgcttct ccaacttcat ctttgaagtg
      481 ccctggtgct tcttcccgaa gtctgtggaa gactgccatt actaaaaccc agctttcttg
      541 tacaaagtgg tgatcctctc gagcctctag aactatagtg agtcgtatta cgtagatcca
      601 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      661 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      721 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcagg
//
