LOCUS       sequence-153935-        2777 bp    DNA     circular SYN 14-APR-2025
DEFINITION  constitutive promoter expressing RFP, biobrick construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Dahl RH, Zhang F, Alonso-Gutierrez J, Baidoo E, Batth TS, 
            Redding-Johanson AM, Petzold CJ, Mukhopadhyay A, Lee TS, Adams PD, 
            Keasling JD
  TITLE     Engineering dynamic pathway regulation using stress-response 
            promoters.
  JOURNAL   Nat Biotechnol. 2013 Nov;31(11):1039-46. doi: 10.1038/nbt.2689. Epub
            2013 Oct 20.
  PUBMED    24142050
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2689"; 
            journalName: "Nat Biotechnol"; date: "2013-11"; volume: "31"; issue:
            "11"; pages: "1039-46"
COMMENT     Sequence Label: pconst #4-RFP
FEATURES             Location/Qualifiers
     source          1..2777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..50
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     CDS             77..754
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      787..858
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      874..901
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(929..946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1063..1608)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1143..1162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1722..1816)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1847..2641)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1958..1977)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2568..2587
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gaattcatga gatcttttac ggctagctca gccctaggta ttatgctagc ggatctttta
       61 agaaggagat atacatatgg cgagtagcga agacgttatc aaagagttca tgcgtttcaa
      121 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      181 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      241 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      301 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      361 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      421 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      481 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      541 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      601 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      661 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
      721 acgtgctgaa ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt aaggatctcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      961 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1021 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1081 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     1201 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1261 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1381 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     1441 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     1501 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     1561 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     1621 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     1681 gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac
     1741 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1801 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt
     1861 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     1921 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     1981 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2041 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2101 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2161 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2221 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2281 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2341 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2401 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     2461 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2521 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2581 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2641 tgcgaaacga tcctcatcct gtctcttgat cagatcatga tcccctgcgc catcagatcc
     2701 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     2761 ccccagctgg caattcc
//
