LOCUS       sequence-153946-         697 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB078d9
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(385..408)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccccgcttca gcggggtttt ttcgccaaaa aaaaccccgc cctgtcaggg gcggggtttt
       61 tttttagggc cctactgact gttagcgttt cggcgccgga taatgcagcg tggtgatcag
      121 accatccgca accgtgctat ggtgatgcac cagttctgcc agggtattga agcgagattc
      181 actagaaacg tacagtttgc catcgctggc ggtgttgata cggtagtgat acacgcgacc
      241 ttcataacgc agagaaatgc tgcgctggcc cgggctactt tcactttcac gaaccagaaa
      301 agaaccatta atgccagagc tcagcaggta ttcggccgcg ttacggctca cagggccatg
      361 ataccagctg tgtttttcca gactcttgtc atcgtcgtcc ttgtagtcta cgaattttac
      421 cctcgcttcc acgacaacac cgataatctt gcagtttccg ttgataggag tcataggcca
      481 tgaaggattc aggcctttca ggtacttctg accgccatct atgaccagtt tcttgaatgt
      541 tgcttcgttc gcgtcagtca gtttggctac aacaaggctt ccattcactg gctcccgtcc
      601 agtatctact aacaccatat gaccttcagg gatgctttga cctacaggtg aggtcatgga
      661 atcaccttca accttcagcc agaatccatt gcctaat
//
