LOCUS       sequence-154010-         881 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors
            with a constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_tBFP_PGK_mCherry
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..810)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(378..397)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     647..665
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat caagcttgca tgcctgcaag
       61 cttgcatgcc tgcaggtcga ctctagagtc gcggccgctt tacttgtaca gctcgtccat
      121 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
      181 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
      241 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
      301 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
      361 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
      421 gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt
      481 caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga
      541 cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat
      601 gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg tcaccttcag
      661 cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc
      721 gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct tgatgatggc
      781 catgttatcc tcctcgccct tgctcaccat ggtaagcttg ggctgcaggt cgaaaggccc
      841 ggagatgagg aagaggagaa cagcgcggca gacgtgcgct t
//
