LOCUS       sequence-154049-         841 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under
            the inducible BAD promoter and a single copy of the endonuclease 
            cleavage site which is readily extendable to multiple copies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Chen Z, Zhao H
  TITLE     A highly sensitive selection method for directed evolution of homing
            endonucleases.
  JOURNAL   Nucleic Acids Res. 2005 Oct 6;33(18):e154.
  PUBMED    16214805
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-10-6"; volume: "33(18)"; pages: "e154"
COMMENT     Sequence Label: p11-LacY-wtx1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcatcgaggc ctttaagcga cttcattcac ctgacgacgc agcagggaaa gcgggcccgg
       61 gccgctaagc gtgaacacgg aaattaaggt gaagcccagc gccaccagac ccagcaccag
      121 ataagcgcct tggaaaccta tgctttcata catattgccc gccagtacag acataaaaat
      181 catcgccagt tgcttaaaga agctgaaact gaccagataa atcgtcgctg aaaaacgcac
      241 ttcaaactga ctagtaatat atttaaagga gcccaccagc aggaacggta cttcaaacat
      301 atgcagcgtt ttcagaataa ccacttccag cgctgaggtg gcgaacgatg agccaataat
      361 acgtacagac ataatagtgc cagccagcag cagggcattc ttcccaccga tgcgattaat
      421 gatcagtggc gcaaagaaca taatcgaggc gttaagtaat tcgcccattg tcgttacgta
      481 gccaaatacg cgggtaccct gttcaccggt agcaaagaac gaagtaaaga aattagcaaa
      541 ctgttggtca aaaacatcgt aggtggagga aacgccaata acatacagtg acaaaaacca
      601 cagttttggc tgtctgaaca gttccagtgc cagcttaagg ctaaatgccg aatggttggc
      661 acctaccgca ttggcaaccg tggcactcga gggcgcgtcc gttttggcga aaaagagtaa
      721 aacggcgagg atgagacaac tgccagagcc cagccagaaa acaaactgat tattaatggt
      781 gaacatgatc ccgacaatcg aggcaaccag cgcccagcca acactgccaa acatccgcgc
      841 g
//
