LOCUS       sequence-154105-         686 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pSB3K3 carrying J105-30gfp-t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Wang B, Barahona M, Buck M
  TITLE     Engineering modular and tunable genetic amplifiers for scaling 
            transcriptional signals in cascaded gene networks.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593.
            Epub 2014 Jul 16.
  PUBMED    25030903
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku593"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9484-92"
COMMENT     Sequence Label: pBW205J105-gfp
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..27
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        28..62
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttctggaatt cgcggccgct tctagagttt acggctagct cagtcctagg tactatgcta
       61 gctactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcnctgg
      121 agttgtccca nttcttgttg aattanatgg tgatgttaat gggcacaaat tttctgtcag
      181 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      241 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      301 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      361 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      421 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttnaaag gtattgattt
      481 taaagaagat ggaaacattc ttggacacna attggaatac aactataact cacacaatgt
      541 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ntagacacna
      601 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      661 tggccctgtc cttttaccag acaacc
//
