LOCUS       sequence-154468-        8065 bp    DNA     circular SYN 14-APR-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS
FEATURES             Location/Qualifiers
     source          1..8065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(907..4305,4306..4308,4309..4905,4906,4907..4909,
                     4910..4911,4912..4914,4915..5007)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(907..930,931..933,934..3420,3421..3423,3424..5007)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5017..5040
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5050..5070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5080..5097
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5120..5137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5126..5350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5421..5437)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5421..5437)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5434..5456)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5445..5461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5469..5475,5476..5493,5494..5499))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5514..5535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5652..5669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5823..6411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5903..5922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6582..7373,7374..7442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7205..7224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7443..7547)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7626..7645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(7817..8065,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gctcattggc
      421 tacgtgcgcg tctcaactaa cgaccagaat accgatcttc agaggaacgc actggtttgt
      481 gcaggctgcg aacagatttt cgaggacaaa ctcagcggga cacggacgga cagacctggc
      541 ctcaagcgag cactcaagag gctgcagaaa ggagacactc tggtggtctg gaaattggac
      601 cgcctgggtc gaagcatgaa gcatctcatt tctctggttg gcgaactgcg agaaaggggg
      661 atcaactttc gaagtctgac ggattccata gatacaagca gccccatggg ccggttcttc
      721 ttctacgtga tgggtgcact ggctgaaatg gaaagagaac tcattataga gcgaaccatg
      781 gcagggcttg cggctgccag gaataaaggc aggcggtttg gaagaccacc aaagggtgga
      841 tccggagggt ccggaggtag tggcggcagc ggtggttcag gtggcagcgg agggtcagga
      901 ggctctgata aaaagtattc tattggttta gctatcggca ctaattccgt tggatgggct
      961 gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg gaacacagac
     1021 cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg cgaaacggca
     1081 gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa gaaccgaata
     1141 tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc tttctttcac
     1201 cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca ccccatcttt
     1261 ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta tcacctcaga
     1321 aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt ggctcttgcc
     1381 catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc ggacaactcg
     1441 gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt tgaagagaac
     1501 cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct ctctaaatcc
     1561 cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg gttgttcggt
     1621 aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt cgacttagct
     1681 gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga caatctactg
     1741 gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct tagcgatgca
     1801 atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc gttatccgct
     1861 tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa ggccctagtc
     1921 cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa aaacgggtac
     1981 gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat caaacccata
     2041 ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga agatctactg
     2101 cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt aggcgaattg
     2161 catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa tcgtgaaaag
     2221 attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc ccgagggaac
     2281 tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg gaattttgag
     2341 gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac caactttgac
     2401 aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga gtatttcaca
     2461 gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa acccgccttt
     2521 ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa ccgcaaagtg
     2581 acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga ttctgtcgag
     2641 atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga cctcctaaag
     2701 ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt agaagatata
     2761 gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact aaaaacatac
     2821 gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta tacgggctgg
     2881 ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg taaaactatt
     2941 ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct gatccatgat
     3001 gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca aggggactca
     3061 ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg catactccag
     3121 acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc ggaaaacatt
     3181 gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa cagtcgagag
     3241 cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt aaaggagcat
     3301 cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct acaaaatgga
     3361 agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta cgacgtcgat
     3421 gccattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt gcttacacgc
     3481 tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt aaagaaaatg
     3541 aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa gttcgataac
     3601 ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt tattaaacgt
     3661 cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga ttcccgaatg
     3721 aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat cactttaaag
     3781 tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag ggagataaat
     3841 aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc actcattaag
     3901 aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta tgacgtccgt
     3961 aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata cttcttttat
     4021 tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga gatacgcaaa
     4081 cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa gggccgggac
     4141 ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa gaaaactgag
     4201 gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag tgataagctc
     4261 atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag ccctacagtt
     4321 gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa actgaagtca
     4381 gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa gaaccccatc
     4441 gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat taaactacca
     4501 aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag cgccggagag
     4561 cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct gtatttagcg
     4621 tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca actttttgtt
     4681 gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt cagtaagaga
     4741 gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa gcacagggat
     4801 aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac caacctcggc
     4861 gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata cacttctacc
     4921 aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata tgaaactcgg
     4981 atagatttgt cacagcttgg gggtgacggt ggctccgatt ataaggatga tgacgacaag
     5041 ggaggttccc caaagaagaa aaggaaggtc tgaaccggtc atcatcacca tcaccattga
     5101 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5161 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5221 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5281 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5341 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc
     5401 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5461 attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg
     5521 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5581 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5641 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5701 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5761 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5821 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6001 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6241 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6421 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6481 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7441 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7501 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7561 aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact
     7621 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7681 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     7741 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     7801 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc
     8041 cacttggcag tacatcaagt gtatc
//
