LOCUS       sequence-154515-         733 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Suicide vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Quandt J, Hynes MF
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria.
  JOURNAL   Gene. 1993 May 15;127(1):15-21.
  PUBMED    8486283
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-05-15"; volume: "127(1)"; pages: "15-21"
COMMENT     Sequence Label: pJQ200KS
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(43..59)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(44..60)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(89..109)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(89..107)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(128..144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(128..144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(141..163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    152..168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(176..182,183..200,201..206))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(501..518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
       61 gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      181 tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc caggggaatt
      301 aattctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
      361 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
      421 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
      481 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
      541 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
      601 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
      661 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
      721 accaggcgtt tcc
//
