LOCUS       sequence-155368-         826 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  AAVS1 TALEN expression vector (Right).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pTALdNC-AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             167..193
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
      121 ctggatcacc atggcttcct cccctccaaa gaaaaagaga aaggttagtt ggaaggacgc
      181 aagtggttgg tctagaatgc atgcggcccc gcgacggcgt gctgcgcaac cctccgacgc
      241 ttcgccggcc gcgcaggtgg atctacgcac gctcggctac agtcagcagc agcaagagaa
      301 gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg tgggccatgg
      361 gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc
      421 tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag acatcgttgg
      481 cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg atgcggggga
      541 gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg caaaacgtgg
      601 cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg gtgcccccct
      661 gaacctgacc ccggaccaag tggtggctat cgccagcaac aatggcggca agcaagcgct
      721 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
      781 agtggtggct atcgccagca acaatggcgg caagcaagcg ctcgaa
//
