LOCUS       sequence-155690-        1473 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway Donor vector containing STK3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STK3_WT
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             654..662
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggagcagc cgccggcgcc taagagtaaa ctaaaaaagc tgagtgaaga cagtttgact
       61 aagcagcctg aagaagtttt tgatgtatta gagaagcttg gagaagggtc ttatggaagt
      121 gtatttaaag caatacacaa ggaatccggt caagttgtcg caattaaaca agtacctgtt
      181 gaatcagatc ttcaggaaat aatcaaagaa atttccataa tgcagcaatg tgacagccca
      241 tatgttgtaa agtactatgg cagttatttt aagaatacag acctctggat tgttatggag
      301 tactgtggcg ctggctctgt ctcagacata attagattac gaaacaagac attaatagaa
      361 gatgaaattg caaccattct taaatctaca ttgaaaggac tagaatattt gcactttatg
      421 agaaaaatac acagagatat aaaagctgga aatattctcc tcaatacaga aggacatgca
      481 aaattggcag attttggagt ggctggtcag ttaacagata caatggcaaa acgcaatact
      541 gtaataggaa ctccattttg gatggctcct gaggtgattc aagaaatagg ctataactgt
      601 gtggccgaca tctggtccct tggcattact tctatagaaa tggctgaagg aaaacctcct
      661 tatgctgata tacatccaat gagggctatt tttatgattc ccacaaatcc accaccaaca
      721 ttcagaaagc cagaactttg gtccgatgat ttcaccgatt ttgttaaaaa gtgtttggtg
      781 aagaatcctg agcagagagc tactgcaaca caacttttac agcatccttt tatcaagaat
      841 gccaaacctg tatcaatatt aagagacctg atcacagaag ctatggagat caaagctaaa
      901 agacatgagg aacagcaacg agaattggaa gaggaagaag aaaattcgga tgaagatgag
      961 ctggattccc acaccatggt gaagactagt gtggagagtg tgggcaccat gcgggccaca
     1021 agcacgatga gtgaaggggc ccagaccatg attgaacata atagcacgat gttggaatcc
     1081 gacttgggga ccatggtgat aaacagtgag gatgaggaag aagaagatgg aactatgaaa
     1141 agaaatgcaa cctcaccaca agtacaaaga ccatctttca tggactactt tgataagcaa
     1201 gacttcaaga ataagagtca cgaaaactgt aatcagaaca tgcatgaacc cttccctatg
     1261 tccaaaaacg tttttcctga taactggaaa gttcctcaag atggagactt tgactttttg
     1321 aaaaatctaa gtttagaaga actacagatg cggttaaaag cactggaccc catgatggaa
     1381 cgggagatag aagaacttcg tcagagatac actgcgaaaa gacagcccat tctggatgcg
     1441 atggatgcaa agaaaagaag gcagcaaaac ttt
//
