LOCUS       sequence-155703-         932 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE76
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      157..409
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        502..681
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        534..717
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(792..811)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
       61 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac
      121 cccaagaaga agaggaaggt gtgagcttgt caagcagatc gttcaaacat ttggcaataa
      181 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
      241 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
      301 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
      361 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg acgctactaa
      421 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
      481 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
      541 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
      601 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
      661 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
      721 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
      781 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      841 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      901 gtgccctgaa tgaactgcag gacgaggcag cg
//
