LOCUS       sequence-155941-       12506 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Vector targeting the mtagBFP2 gene to the GAPDH locus of human 
            cells. BFP is expressed from a 2A sequence at the C terminus of 
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mtagBFP2-IRESMuro
FEATURES             Location/Qualifiers
     source          1..12506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5635..6336
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    join(6353..6927,6928..6930,6931..6939)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6520..6537)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6747..6766
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(11862..11881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11863..11881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11891..11908)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11891..11907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11900..11922)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12048..12503)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12185..12206)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12397..12416
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccgatgagc
     5641 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     5701 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     5761 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     5821 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     5881 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     5941 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     6001 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     6061 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     6121 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     6181 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     6241 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     6301 gacctcccta gcaaactggg gcacaagctt aattgacaag tcgacggatc cgcccctctc
     6361 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6421 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6481 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6541 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     6601 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     6661 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6721 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6781 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     6841 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6901 ttttcctttg aaaaacacga tgataatatg gccacaacca tggcgcgtat ggcaagactg
     6961 accgaataca agcctaccgt gagactggca acaagagatg atgtcccaag agccgtgcga
     7021 accctggcag cagcattcgc agactaccca gccacaagac acactgtgga ccccgatagg
     7081 catatcgaga gagtgaccga gctgcaggaa ctgtttctga cacgggtggg cctggatatt
     7141 ggaaaggtct gggtggcaga tgatggggct gctgtggccg tctggacaac cccagagtct
     7201 gtggaagctg gagctgtctt cgctgagatc ggccctcgaa tggcagaact gtctggctcc
     7261 aggctggcag cacagcagca gatggaggga ctgctggcac cacacagacc taaggaacca
     7321 gcttggttcc tggcaactgt gggggtctct cccgaccatc agggcaaagg actggggagt
     7381 gccgtggtcc tgcctggagt ggaggctgca gaaagagctg gagtcccagc ttttctggag
     7441 acatctgccc cccggaacct gcctttctat gaacgcctgg gctttactgt gaccgctgac
     7501 gtggaggtgc ccgaaggccc ccgaacttgg tgcatgaccc gaaagccagg agcataaatc
     7561 tagaggcgcg ccatcgataa gcttagatcg agcacaagag gaagagagag accctcactg
     7621 ctggggagtc cctgccacac tcagtccccc accacactga atctcccctc ctcacagttg
     7681 ccatgtagac cccttgaaga ggggaggggc ctagggagcc gcaccttgtc atgtaccatc
     7741 aataaagtac cctgtgctca accagttact tgtcctgtct tattctaggg tctggggcag
     7801 aggggaggga agctgggctt gtgtcaaggt gagacattct tgctggggag ggacctggta
     7861 tgttctcctc agactgaggg tagggcctcc aaacagcctt gcttgcttcg agaaccattt
     7921 gcttcccgct cagacgtctt gagtgctaca ggaagctggc accactactt cagagaacaa
     7981 ggccttttcc tctcctcgct ccagtcctag gctatctgct gttggccaaa catggaagaa
     8041 gctattctgt gggcagcccc agggaggctg acaggtggag gaagtcaggg ctcgcactgg
     8101 gctctgacgc tgactggtta gtggagctca gcctggagct gagctgcagc gggcaattcc
     8161 agcttggcct ccgcagctgt gaggtcttga gcacgtgctc tattgctttc tgtgccctcg
     8221 tgtcttatct gaggacatcg tggccagccc ctaaggtctt caagcaggat tcatctaggt
     8281 aaaccaagta cctaaaacca tgcccaaggc ggtaaggact atataatgtt taaaaatcgg
     8341 taaaaatgcc cacctcgcat agttttgagg aagatgaact gagatgtgtc agggtgactt
     8401 atttccatca tcgtccttag gggaacttgg gtaggggcaa ggcgtgtagc tgggacctag
     8461 gtccagaccc ctggctctgc cactgaacgg ctcagttgct ttgggcagtt actcccgggc
     8521 ctcactttgc acgtgtgctt acctagtgga gacaaaagta catacctcgg tagagcgcgc
     8581 acgcctgtaa ccccagcact ttgggaggcc aaggtgggtg tatcacctga ggtcaggagt
     8641 ttgagaccag cctggccaac atggtgaaac tccgtctcta ctaaaattac aaaaatcagc
     8701 caggcttcat ggcacatgcc tatagtccca gctacaggca tgctgaagca ggagaatcgc
     8761 ttgcaccccg gaggcagagg ctgcagtgag ctgagaccac accactgcac tccagcctag
     8821 gcaacagagt atgagactcc atctcaaaaa aaaaaaaagt acctacctca gagttcaaac
     8881 tagtgaatat taggaagtgc ttgagacagt gacaccaaag tgcacaataa atactcgcca
     8941 gtttcattat tattaaagaa tccatttgaa tgtcagctca acacagcctc ctataccgag
     9001 gcattgtgaa ccgcatctcc ccagcttctc caggcttttc caagaatcag ggacactgta
     9061 gcctgttggt ctcagtgtat gacagacacg gaggaagcac atctttagct gatacttaaa
     9121 cagagaccct gagcgcacat acacccgcgc acacatgcat ggagcttcac cttctctgtc
     9181 attctgcagt gaccaggaga gcaagagctc ccacctccct tcaaaacact gtgcccatcc
     9241 cgggcactaa ggcctcttta aagcacggca cctccacgag ggagggccac agccacatac
     9301 actccacctg gcaggtggac agcgtgagca cgtggaccat agcagggaca aggtgccccg
     9361 gccagcccca acgccctctg ccgctgacag ggacagaagc cctctccagc tgcgtgtgct
     9421 gcagaggcca tgcgtagcct ccagctgcat tctattccac tccagtgcct gggccagtta
     9481 gcaccagtgt ggaagacagt gagctggctc cggacaacag ggatggagga aaggtcccac
     9541 attcacattc ctgatacgtg gacaaggtga ggggccgcaa tcgctctggc agcattttaa
     9601 agatggggaa gtagcagaca cccacgcgtg aaggcaggag agccccaact gtggtggaaa
     9661 tggccccaga atggtagggc caagcctagc tccagacacc ccagagccct ggagaagcca
     9721 agactgaggg agaaagcctg agggaggagc gccccagtcc ccagggaccg gcctggtgca
     9781 gagctgcagc tgatgttccc ctctgtgcag ccccaccctc tgcctcgctg agctccctgc
     9841 tgcgagggcc tcgggtgcaa gggggaggca ggtctctatc tcatggagct gtcagatgag
     9901 acatcgcgat cggagtcctc agcctcgctt ggcggcggcg gcgggtcgct aagcgggacc
     9961 gcagtgaaag caggagactt tctagaaaaa aacaccagtt gtcaaccttg gggcaggcag
    10021 gaatcctgaa gacggacggc actcctcctc ctgctgcctc accctctggc agcccgtgag
    10081 aagtaccgga agcgagggcg gggccgcggg atggcgaggg agcggcaggg actgaactct
    10141 ctccaaaccc accctgacag ggaaatgggc cccgcctgtg tcttgggaac tcagaggctg
    10201 aggtcaggca tgatggctca cgcctgtaat tccagcactt tgggaggcag aggcgggtgg
    10261 atcacgacgt caggagttca aggcaagcct ggccaacatg gtgaaacccc atctctacta
    10321 aaaatgcaaa aattagccag gtgtggtggc gggcgccttg gaggctgagg caagataatc
    10381 acttgaacct gggaggcgga ggttgtagtg agccaaaaaa aaaaaaaaaa gaaatagctg
    10441 aagtcacagt aggagagaag ctgctgagcc tccagcaccc tgactctagg gccttggctt
    10501 tatgtctatc tgcagtattt ttgtgatttt taaaaattca ctttcttgtt gcggtgtaac
    10561 ttacacaggg tcaaatgcac aaatcatggc cctgacttta gataaaaatc tgcccccaca
    10621 acccttctgt tccttgccag tttttaaact gcctctaacc aggggaacca ccagagctgg
    10681 tggccttggg aggtttcagc cctcccgtca tgaatggaca tagctcatcc aactgccaag
    10741 ggagagagct gtgggtctgg gccagcccca ccaggtaact cccaaagggc agccccacag
    10801 caagatgtga cccagtcatt gcctgagggt ctctggggct gtgttccaac ctttctcccc
    10861 gctgtgtccc cctggaaggc cccatgccca ggggaggcgc ctaccggtct ccagactggg
    10921 tgatgagccg gcggcaggtc tccatctgca cgtccaggcc gcgcttcatg ctgcacatct
    10981 ccatgtactc gtgcaggtgc cggttcatgt cgttcttggc cgtggccaac tccagctgtg
    11041 gtggggcagg cagggccatc ggggttaaca ggtggcgttc acagcgcctc tgttgccccc
    11101 gccaggaggc caacacgcca agagcagtgg ctgggccggg ggcccaggca gccattactg
    11161 aaggctcaga ttttaaaata aaccagacca aggcagaagg cagcgaatac accattgttt
    11221 cctctgcttt tcctggaaat ctttgcctat ggaggaatgc aaaggtactg ctgcaggctg
    11281 tgttctttta agactgataa gagtggtgag gaaagtcagt gtgggcggga tggagcagat
    11341 ccacaggctc ccagggccac acaggttcct atggggcccg gctcacccct catcccctgt
    11401 actgcgctgg tcctgggctg tcccctcact gcactgtgag ctcccgtaag ggttagcgac
    11461 aggttttcct catcctggca gacccagacc tcgaagggaa ttaactctct ctgtgacccg
    11521 tggcatacaa gcctggctaa ctgctgaaag gggcccaatc ctcttaccat tctctcctta
    11581 tcactttatt ttctcaccta aaagccattt tcagacacta aaaggaagac tcccatattg
    11641 gaggtgccat tgattctgag acctgtcctg atttcagaaa tgtcacacgt ggaaaacacg
    11701 gggctcagaa tcaaagaaat ccggtctccc tccccgggcc cattctgcag cccacactct
    11761 gaggcaggcg gagaggcagg gcccaggcag cacaaactca gtgccaggga cagtcgatta
    11821 attaagcgat cgcaggccgc ccgcggtgga gctccaattc gccctatagt gagtcgtatt
    11881 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
    11941 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
    12001 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
    12061 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
    12121 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    12181 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    12241 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    12301 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    12361 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    12421 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    12481 taatgcgccg ctacagggcg cgtcag
//
