LOCUS       sequence-155949-         828 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro3GFP
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             112..825
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHCVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLCTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(142..170)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     765..785
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcccc ccgctgaatt catgagtaaa
      121 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      181 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      241 cttaaattta tttccactac tggaaaacta cctgttccat ggccaacact tgtcactact
      301 ttcagttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt
      361 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      421 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      481 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      541 aactataact cacactgtgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      601 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      661 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtgcaca
      721 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      781 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataa
//
