LOCUS       sequence-156117-         825 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of STK16. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK16_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gtcatcattg acaataagca ctacctcttc atccagaaac
      181 tgggggaggg tgggttcagc tatgtggacc tagtggaagg gttacatgat ggacacttct
      241 acgccctgaa gcgaatcctg tgtcacgagc agcaggaccg ggaggaggcc cagcgagaag
      301 ccgacatgca tcgcctcttc aatcacccca acatccttcg cctcgtggct tactgtctga
      361 gggaacgggg tgctaagcat gaggcctggc tgctgctacc attcttcaag agaggtacgc
      421 tgtggaatga gatagaaagg ctgaaggaca aaggcaactt cctgaccgag gatcaaatcc
      481 tttggctgct gctggggatc tgcagaggcc ttgaggccat tcatgccaag ggttatgccc
      541 acagagactt gaagcccacc aatatattgc ttggagatga ggggcagcca gttttaatgg
      601 acttgggttc catgaatcaa gcatgcatcc atgtggaggg ctcccgccag gctctgaccc
      661 tgcaggactg ggcagcccag cggtgcacca tctcctaccg agccccagag ctcttctctg
      721 tgcagagtca ctgtgtcatc gatgagcgga ctgatgtctg gtccctaggc tgcgtgctat
      781 atgccatgat gtttggggaa ggcccttatg acatggtgtt ccaaa
//
