LOCUS       sequence-156133-         966 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian GFP reporter plasmid, target site for Vika recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Karimova M, Abi-Ghanem J, Berger N, Surendranath V, Pisabarro MT, 
            Buchholz F
  TITLE     Vika/vox, a novel efficient and specific Cre/loxP-like site-specific
            recombination system.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):e37. doi: 10.1093/nar/gks1037. 
            Epub 2012 Nov 9.
  PUBMED    23143104
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-01"; volume: "41(2):e37. doi"; pages: " 
            10.1093/nar/gks1037. Epub 2012 Nov 9"
COMMENT     Sequence Label: pRK5-vox-EGFP
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(176..964)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRCQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(284..303)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     894..913
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
       61 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
      121 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gcccagaaga
      181 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
      241 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
      301 acgctatgtc ctgacagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
      361 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
      421 cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
      481 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
      541 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
      601 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
      661 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
      721 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
      781 cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
      841 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
      901 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
      961 caatca
//
