LOCUS       sequence-156196-         852 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of FGFR3. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: FGFR3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca cccacgctgg ccaatgtctc cgagctcgag ctgcctgccg
      181 accccaaatg ggagctgtct cgggcccggc tgaccctggg caagcccctt ggggagggct
      241 gcttcggcca ggtggtcatg gcggaggcca tcggcattga caaggaccgg gccgccaagc
      301 ctgtcaccgt agccgtgaag atgctgaaag acgatgccac tgacaaggac ctgtcggacc
      361 tggtgtctga gatggagatg atgaagatga tcgggaaaca caaaaacatc atcaacctgc
      421 tgggcgcctg cacgcagggc gggcccctgt acgtgctggt ggagtacgcg gccaagggta
      481 acctgcggga gtttctgcgg gcgcggcggc ccccgggcct ggactactcc ttcgacacct
      541 gcaagccgcc cgaggagcag ctcaccttca aggacctggt gtcctgtgcc taccaggtgg
      601 cccggggcat ggagtacttg gcctcccaga agtgcatcca cagggacctg gctgcccgca
      661 atgtgctggt gaccgaggac aacgtgatga agatcgcaga cttcgggctg gcccgggacg
      721 tgcacaacct cgactactac aagaagacaa ccaacggccg gctgcccgtg aagtggatgg
      781 cgcctgaggc cttgtttgac cgagtctaca ctcaccagag tgacgtctgg tcctttgggg
      841 tcctgctctg gg
//
