LOCUS       sequence-156390-         858 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knocking-in EGFP 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-EGFP
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(58..771,772..774,775..777))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(61..771,772..774,775..777))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(103..124)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     452..471
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     711..732
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(820..839)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcac gcgttcatta
       61 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      121 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      181 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      241 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      301 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      361 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      421 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      481 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      541 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
      601 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      661 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      721 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
      781 ggcggcgtcg acgaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc
      841 caggagctgt aggaaaaa
//
