LOCUS       sequence-156440-         885 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of EPHB2. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: EPHB2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gaccccaacg aggcagtgcg ggagtttgcc aaggaaattg
      181 acatctcctg tgtcaaaatt gagcaggtga tcggagcagg ggagtttggc gaggtctgca
      241 gtggccacct gaagctgcca ggcaagagag agatctttgt ggccatcaag acgctcaagt
      301 cgggctacac ggagaagcag cgccgggact tcctgagcga agcctccatc atgggccagt
      361 tcgaccatcc caacgtcatc cacctggagg gtgtcgtgac caagagcaca cctgtgatga
      421 tcatcaccga gttcatggag aatggctccc tggactcctt tctccggcaa aacgatgggc
      481 agttcacagt catccagctg gtgggcatgc ttcggggcat cgcagctggc atgaagtacc
      541 tggcagacat gaactatgtt caccgtgacc tggctgcccg caacatcctc gtcaacagca
      601 acctggtctg caaggtgtcg gactttgggc tctcacgctt tctagaggac gatacctcag
      661 accccaccta caccagtgcc ctgggcggaa agatccccat ccgctggaca gccccggaag
      721 ccatccagta ccggaagttc acctcggcca gtgatgtgtg gagctacggc attgtcatgt
      781 gggaggtgat gtcctatggg gagcggccct actgggacat gaccaaccag gatgtaatca
      841 atgccattga gcgggactat cggctgccac cgcccatgga ctgcc
//
