LOCUS       sequence-156499-        1070 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  retroviral expression of Gata3-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lazarus Gata 3 flag
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..30)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(165..677,678..680,681..683))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     358..377
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     617..638
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(857..876)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
       61 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      121 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      181 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      241 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      301 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      361 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      421 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      481 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      541 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      601 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      661 agctcctcgc ccttgctcac catggttgtg gccatattat catcgtgttt ttcaaaggaa
      721 aaccacgtcc ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt
      781 aaagcatgtg caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc
      841 cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca
      901 actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt
      961 ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac
     1021 agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
//
