LOCUS       sequence-156602-         882 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression of SdeA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Qiu J, Sheedlo MJ, Yu K, Tan Y, Nakayasu ES, Das C, Liu X, Luo ZQ
  TITLE     Ubiquitination independent of E1 and E2 enzymes by bacterial 
            effectors.
  JOURNAL   Nature. 2016 May 5;533(7601):120-4. doi: 10.1038/nature17657. Epub 
            2016 Apr 6.
  PUBMED    27049943
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17657"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "120-4"
COMMENT     Sequence Label: pQE30-SdeA
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             46..57
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttatttgct ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta
       61 actatgagag gatcgcatca ccatcaccat cacggatcca tcagtttggg agaagccata
      121 atgcctaagt atgtcgaagg ggtagaacta actcaagaag gtatgcatgc tatttttgca
      181 cgcatggggt atggagatat taccagcggt agcatataca acggagtgcc aactattgac
      241 acaggtgcgc taaacaggca aggttttatg cccgtgttaa ccggggtagg accacacaga
      301 gactccggcc actggattat gttaatcaaa ggtcctggca accaatacta cctctttgac
      361 ccattaggca aaacatcagg tgaaggctat caaaatattt tagcagccca attaccaatg
      421 gggtctaccc tttctgttat tcccaatggt tccggtttga atatgggatt atgcggctac
      481 tgggtcgcct ctgctggttt aagagcacac caggcattaa atcaacataa tccccctact
      541 ttgttaaatg tgggacaaac cattaccaat gaaatgcgta acgaactcga tcatgacggt
      601 tatcgaaaaa ttaccggatg gttacgtgct gttgctgatg aattccctga aggtgaccca
      661 caattagatg ggaaagcatt aagagaaaac actgaaaaag atttaaaaat agaaattcct
      721 acactggttc ttcctggaaa agatacctcc cctaaagaaa tgtctgttaa acccactgca
      781 ccacaagata aatctgtgcc tgtttggaat gggttttcat tatatactga tgataccgtt
      841 aaagccgcgg cacaatacgc ctatgacaac tatctgggca aa
//
