LOCUS       sequence-156646-        1146 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Rodriguez EA, Tran GN, Gross LA, Crisp JL, Shu X, Lin JY, Tsien RY
  TITLE     A far-red fluorescent protein evolved from a cyanobacterial 
            phycobiliprotein.
  JOURNAL   Nat Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935.
  PUBMED    27479328
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Aug 1. doi: 10.1038/nmeth.3935."
COMMENT     Sequence Label: pBAD-TDsmURFP-RBS-HO-1
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             950..967
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcaactctc tactgtttct cgatacccgt ttttttgggc tagaaataat tttgtttaac
       61 tttaagaagg agatatggat ccatgaaaac ttctgaacaa cgtgtaaaca tcgcaactct
      121 gctgactgag aacaaaaaga aaatcgttga taaggcgtct caggatctgt ggcgtcgtca
      181 tccagatctc attgcgccgg gcggcatcgc ttttagtcaa cgcgatcgtg cactgtgcct
      241 gcgtgactac ggctggtttc tgcacctgat cacgttctgt ctgctggctg gtgataaagg
      301 tcctattgaa tccatcggtc taattagcat acgcgaaatg tacaacagcc tgggtgtgcc
      361 ggttccggct atgatggaat ctatccgttg tctgaaagag gcctccctgt cactgctgga
      421 cgaggaagac gccaatgaga ctgcaccgta ctttgactat atcatcaagg ctatgtccgg
      481 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa
      541 catggccatg aaaacttctg aacaacgtgt aaacatcgca actctgctga ctgagaacaa
      601 aaagaaaatc gttgataagg cgtctcagga tctgtggcgt cgtcatccag atctcattgc
      661 gccgggcggc atcgctttta gtcaacgcga tcgtgcactg tgcctgcgtg actacggctg
      721 gtttctgcac ctgatcacgt tctgtctgct ggctggtgat aaaggtccta ttgaatccat
      781 cggtctaatt agcatacgcg aaatgtacaa cagcctgggt gtgccggttc cggctatgat
      841 ggaatctatc cgttgtctga aagaggcctc cctgtcactg ctggacgagg aagacgccaa
      901 tgagactgca ccgtactttg actatatcat caaggctatg tccgaattcc atcatcacca
      961 tcaccattga aagaaggaga tatacatacc catgagtgtc aacttagctt cccagttgcg
     1021 ggaagggacg aaaaaatccc actccatggc ggagaacgtc ggctttgtca aatgcttcct
     1081 caagggcgtt gtcgagaaaa attcctaccg taagctggtt ggcaatctct actttgtcta
     1141 cagtgc
//
