LOCUS       sequence-156767-        1003 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression of Human catalytic mutant SIRT5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Kumar S, Lombard DB
  TITLE     Generation and Purification of Catalytically Active Recombinant 
            Sirtuin5 (SIRT5) Protein.
  JOURNAL   Methods Mol Biol. 2016;1436:241-57. doi: 
            10.1007/978-1-4939-3667-0_16.
  PUBMED    27246219
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1436:241-57. doi"; pages: " 
            10.1007/978-1-4939-3667-0_16"
COMMENT     Sequence Label: SIRT5H158Y-pET15b
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(865..882)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(892..909)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             928..950
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    965..989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcctc gagttaagaa acagtttcat tttcatgaca
       61 ggcaagggct tcaggaagag tcgttccaca gggtccctgg aaatgaaacc tgaatctgtt
      121 cgtagctggg gtggtctccg tgttaaattc agccactggc acgcccctgg cagccacctg
      181 gggggcaaac atggctgctg ggtacaccac agaggaagtg cccaccacta gacataaatc
      241 acagtgggcg agctctctgt caacctcctc cagaatggca ggatccaggt tttctccaaa
      301 ccacacgacg tgaggtcgca gcaagccccc gcagcctgcc tcttcacacc ggggaagttt
      361 ctcaactggg atgctggcat cttgagttcc aggttctgga gcaccttttc ctgataaagc
      421 tggacaaatt ggactcttgt aattctcagc cacaactcca caagaggtac atcgagtttt
      481 aaataagcta ccatagatct ccagaaggtt cttggtgcca gccttgcggt gcagctcatc
      541 gatgttctgg gtgatgacca cgactcgccg gccctgcttg cccagccggg tctcacactc
      601 ggctatggcg cggtgcccgg cgttgggctc cttgctcccc atgacctccc gccggtagtg
      661 gtagaactcc cacacccggg acgggttgtg ggcaaaggcc aggggagtcg ccaggtcctg
      721 ggcttgccat tttctccaat aacctccagc tcctctgaag gtcggaacac cactttctgc
      781 actaacacca gctcctgaga tgatgactat gtgctttgct tttgcaaaaa actttcgaaa
      841 atctgccata cttgaactca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg
      901 atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa attatttcta
      961 gaggggaatt gttatccgct cacaattccc ctatagtgag tcg
//
