LOCUS       sequence-156790-       13868 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentivirus vector. Expresses Halotag-Cbx7 fusion proteins in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13868)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-HT-Cbx7
FEATURES             Location/Qualifiers
     source          1..13868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3014..3904
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     promoter        4686..5897
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5734..5753
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5916..6623
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    6646..7218
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6813..6830)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7040..7059
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7221..7823
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7720..7740
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7839..8427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7892..7912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8310..8321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8634..8867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8968..8985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8974..9198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9244..9672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9331..9350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9541..9562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9681..9701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9686..10015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9866..10001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9928..9947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11223..11344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11393..11409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11393..11409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11406..11428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11417..11433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11441..11447,11448..11465,11466..11471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11486..11507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11624..11641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11795..12383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11875..11894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12554..13345,13346..13414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13177..13196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13415..13519)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13567..13701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtccg
     3001 ctcgagggcc accatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga
     3061 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     3121 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     3181 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     3241 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     3301 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     3361 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     3421 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     3481 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     3541 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     3601 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     3661 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     3721 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     3781 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     3841 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     3901 cggcgcgaat tcacttgcta tggagctgtc agccatcggc gagcaggtgt tcgccgtgga
     3961 gagcatccgg aagaagcgcg tgcggaaggg taaagtcgag tatctggtga agtggaaagg
     4021 atggccccca aagtacagca cgtgggagcc agaagagcac atcttggacc cccgcctcgt
     4081 catggcctac gaggagaagg aggagagaga ccgagcatcg gggtatagga agagaggtcc
     4141 gaaacccaag cggcttctgc tgcagcggct gtacagcatg gacctgcgga gctcccacaa
     4201 ggccaagggc aaggagaagc tctgcttctc cctgacgtgc ccactcggca gcgggagccc
     4261 tgagggggtg gtcaaggcgg gggcacctga gctggtggac aagggcccct tggtgcccac
     4321 cctgcccttc ccgctccgca agccccgaaa ggcccacaag tacctgcggc tctcgcgcaa
     4381 gaagttcccg ccccgcgggc ccaacctgga gagccacagc catcgacggg agctcttcct
     4441 gcaggagcca ccggccccag acgtcctgca ggcggctggc gagtgggagc ctgctgcgca
     4501 gccccctgaa gaggaggcag atgccgacct ggccgagggg ccccctccct ggacacctgc
     4561 gctcccctca agtgaggtga ccgtgaccga catcaccgcc aactccatca ccgtcacctt
     4621 ccgcgaggcc caggcagctg agggcttctt ccgagaccgc agtgggaagt tctgagcgga
     4681 cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     4741 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
     4801 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
     4861 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
     4921 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
     4981 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
     5041 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
     5101 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
     5161 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
     5221 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
     5281 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
     5341 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     5401 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     5461 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     5521 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     5581 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     5641 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     5701 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     5761 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     5821 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     5881 tggctttttt gttagacgct agcggatccg ccaccatgtc taggctggac aagagcaaag
     5941 tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga
     6001 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     6061 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     6121 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     6181 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     6241 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     6301 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     6361 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     6421 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     6481 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     6541 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgattttg
     6601 accttgacat gctccccggg taaggatccg cggccgcaaa ttccgcccct ctccctcccc
     6661 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     6721 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     6781 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     6841 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     6901 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     6961 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     7021 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7081 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7141 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     7201 ttgaaaaaca cgataatacc atggccaccg agtacaagcc cacggtgcgc ctcgccaccc
     7261 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     7321 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7381 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7441 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggct
     7501 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7561 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7621 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7681 gcgctggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7741 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7801 tgacccgcaa gcccggtgcc tgagttcgcg tctggaacaa tcaacctctg gattacaaaa
     7861 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7921 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7981 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8041 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8101 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8161 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8221 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     8281 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8341 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8401 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     8461 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     8521 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     8581 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     8641 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8701 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8761 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8821 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8881 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8941 ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     9001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     9181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     9241 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9661 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10021 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact
    10081 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
    10141 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
    10201 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
    10261 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat taattcagcg
    10321 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
    10381 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
    10441 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
    10501 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
    10561 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
    10621 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
    10681 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
    10741 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
    10801 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
    10861 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
    10921 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    10981 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    11041 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc
    11101 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    11161 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    11221 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11341 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    11401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11461 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    11641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    11761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    11821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    11881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    11941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    12181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    12241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    12301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    12421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    12481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    12541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    12601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    12661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    12721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    12781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    12841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    12901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    12961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    13021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    13081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    13141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    13201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    13261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    13321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    13381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    13441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    13501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    13561 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    13621 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    13681 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    13741 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    13801 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    13861 ttagtagt
//
