LOCUS       sequence-156850-         778 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Non-integrating (episomal) expression of human C-MYC, LIN28 and 
            NANOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hMLN
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..96)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 actcatggtg aagggcgaat tgttcatgag tggacctgga ttgctttcta catccccagc
       61 cagtttgagt aaatcaaagt taagagtttg tttgacagga gcgacaattt tgagatcccc
      121 attctgtgcc tccgggagca gggtagggct gtggatttct tcttcttcct ctcgaaagta
      181 ggttggcttt ccctgtgcac tagggccctg ctgggccttc agcggacatg aggctaccat
      241 atggctgatg ctctggcaga agtggcactt cttgggctgg ggtggcagct tgcattcctt
      301 ggcatgatga tctagacctc cacagttgta gcacctgtct ccttttgatc tgcgcttctg
      361 catgctcttt ccttttggcc gcctctcact cccaatacag aatactccac caggtccggt
      421 gacacggatg gattccagac ccttggctga cttcttaaag gtgaactcca ctgcctcacc
      481 ctccttcaag ctccggaacc cttccatgtg cagcttactc tggtgcacaa agacatccac
      541 tggggggtcg agcgcgaccc cggcgcgggc ggtcatggac aggaagccga accccatgcg
      601 cacgttgaac cacttacaga tgcccgcacc gtgcagcagc tgaggctcgt ccgccgcccg
      661 ggccgcgtcc tccggcgcct cctcgggcgc ctcttctgcc gccttggcgc agccacctgc
      721 aaactgctgg ttggacacgg agcccatgag tggacctgga ttgctttcta catcccca
//
