LOCUS       sequence-156957-         994 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expression of Zdk1 tagged RhoA Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-mVenus-Zdk1-RhoA Q63L
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..93)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(100..132)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(join(169..561,562..564,565..750))
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (Q63L)
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGLEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     CDS             complement(781..957)
                     /codon_start=1
                     /product="""Zdark"" protein, derived from the Z subunit of 
                     protein A, that binds to the dark state of LOV2 and is 
                     released upon irradiation (Wang et al., 2016)"
                     /label=Zdk1
                     /translation="MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANL
                     LAEAKKLNDAQAPK"
ORIGIN
        1 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggtt
       61 aatcacttag tgatggtgat ggtgatggtg gtgctcgaga tcctcggggt cttccggggc
      121 gagttctggc tggcttgcac gtgtatacag ctgtgcggcc gcaagctttt acaagacaag
      181 gcacccagat tttttcttcc cacgtctagc ttgcagagca gctctcgtag ccatttcaaa
      241 aacctctctc actccatctt tggtctttgc tgaacactcc atgtacccaa aagcgccaat
      301 cctgtttgcc atatctctgc cttcttcagg tttcaccggc tcctgcttca tcttggctag
      361 ctcccgcctt gtgtgctcat cattccgaag atccttctta ttcccaacca ggatgatggg
      421 cacgttggga cagaaatgct tgacttctgg ggtccacttt tctgggatgt tttctaaact
      481 atcagggctg tcgatggaaa aacacatcag tataacatcg gtatctgggt aggagagggg
      541 cctcaggcga tcataatctt ccagcccagc tgtgtcccac aaagccaact ctacctgctt
      601 tccatccacc tcgatatctg ccacatagtt ctcaaacact gtgggcacat acacctctgg
      661 gaactggtcc ttgctgaaga ctatgagcaa gcatgtcttt ccacaggctc catcaccaac
      721 aatcaccagt ttcttccgga tggcagccat ggtaccacca gaaccagaac caccggatcc
      781 ttttggggcc tgggcatcgt tcagtttttt ggcttcggcc agcagattgg cgctctgaga
      841 tggatcatca aacaggctca cgatgaaggc aaacttctgc tcaacattca ggtttggcag
      901 agaatggatt tccgcaccgg cacgcgtctt ttctttattg aatttgttat ccaccataga
      961 tccacttcca gaacccttgt acagctcgtc catg
//
