LOCUS       sequence-156969-         912 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting human telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1651-sgTelomere(F+E)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(133..843)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(411..430)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     680..698
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(856..909)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
      181 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
      241 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
      301 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
      361 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
      421 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
      481 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
      541 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
      601 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
      661 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
      721 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
      781 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
      841 catggtggcg accggtgggc caggattctc ctcgacgtca ccgcatgtta gcagacttcc
      901 tctgccctct cc
//
