LOCUS       sequence-157077-         993 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gateway entry vector encoding shRNA to UBR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, 
            Stokke T, Patzke S, Saunders DN
  TITLE     The E3 ubiquitin ligase UBR5 regulates centriolar satellite 
            stability and primary cilia.
  JOURNAL   Mol Biol Cell. 2018 May 9:mbcE17040248. doi: 
            10.1091/mbc.E17-04-0248.
  PUBMED    29742019
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2018 May 9:mbcE17040248. doi: 10.1091/mbc.E17-04-0248."
COMMENT     Sequence Label: pEN_TmiRc3_shURB5
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(147..273)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(301..315)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(343..464)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(606..630)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(627..665)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(698..716,717..739,740..758,759..781,782..800,801..823,
                     824..842,843..865,866..884,885..907,908..926,927..949,
                     950..968)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtct agatatctcg
      121 aaaacgcatt agtcttccaa ttgaaaaaag tgatttaatt tataccattt taattcagct
      181 ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa gataattgct
      241 cctaaagtag ccccttgaat tccgaggcag taggcaagca gtgaatgtag attccaaata
      301 catctgtggc ttcactattt ggaatctaca ttcactgcgc gctcactgtc aacagcaata
      361 taccttctcg agccttctgt tgggttaacc tgaagaagta atcccagcaa gtgtttccaa
      421 gatgtgcagg caacgattct gtaaagtact gaagcctcat tcaaacaatt accctgttat
      481 ccctagtcga ctaggactag agtcactagt gatcagacgc ctaggcctag ttcggggccg
      541 cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc
      601 caggcgatct gacggttcac taaacgagct ctgcttatat aggcctccca ccgtacacgc
      661 ctacctcgac ccgggtaccg agctcgactt tcacttttct ctatcactga tagggagtgg
      721 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
      781 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      841 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
      901 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
      961 gatagggagt ggtaaaggat ccagtcgact gaa
//
