LOCUS       sequence-157828-         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mammalian expression of Smad7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Moustakas plasmids
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-Smad7
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccaccgc catggactac aaggacgatg atgacaaggg
      121 cgaattcggg ttcaggacca aacgatctgc gctcgtccgg cgtctctgga ggagccgtgc
      181 gcccggcggc gaggacgagg aggagggcgt ggggggtggc ggcggaggag gcgagctgcg
      241 gggagaaggg gcgacggacg gccgggctta tggggctggt ggcggcggtg cgggcagggc
      301 tggctgctgc ctgggcaagg cagtccgagg tgccaaaggt caccaccatc cccatccccc
      361 aacctcgggt gccggggcgg ccgggggcgc cgaggcggat ctgaaggcgc tcacgcactc
      421 ggtgctcaag aaactcaagg agcggcagct ggagctgctg cttcaggccg tggagtcccg
      481 cggcggtacg cgcaccgcgt gcctcctgct gcccggccgc ctggactgca ggctgggccc
      541 gggggcgccc gccagcgcgc agcccgcgca gccgccctcg tcctactcgc tccccctcct
      601 gctgtgcaaa gtgttcaggt ggccggatct caggcattcc tcggaagtca agaggctgtg
      661 ttgctgtgaa tcttacggga agatcaaccc cgagctggtg tgctgcaacc cccatcacct
      721 tagtcgactc tgtgaactag agtctccccc tcctccttac tccagatacc caatggattt
      781 tctcaaacca actgcaggct gtccagatgc tgtaccttcc tccgcggaaa cc
//
