LOCUS       sequence-157934-         956 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Ubiquitin promoter driving Avi-tagged protein containing Cerulean 
            protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 
            2012); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-Avi-Cerulean-Rpl10a
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(53..97)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 gctcctcgcc cttgctcacc attcctccct ggaagtacag gttctctcct ccctcatgcc
       61 actcgatctt ctgggcctca aagatgtcat tcaggccacc agccatctgt aaacaaattc
      121 aaagtaagat tagcaatcac acatttccat ttaaaaaata ctacaatgtt ctagccaata
      181 atgtatgttt tgcacttctc ccatgttaaa cgagatagtt cacccgaaaa tgaaatttta
      241 ctttgcattt ggtcaccctt aaatgttaca aacctttaaa ataatagaaa actattgact
      301 atacttgtag agaaaaataa atactatcca aaacaatggc tgacagtgga ctatagaatg
      361 cattttacct gcagcaaaat tgtccaaatg atcctatatg aaaatttagg gtcttaccta
      421 gtgaaatgat agctcatttt gcacaagctc tgagatttgg ctttgtgcat catgaaatct
      481 aattatgtca tgcatttcca aagcattggc cattttaaac aaactgcaca tctgtgcagt
      541 ttacattgtt ttaaaataaa aaataaaaaa ttaagaaata aagaataata atgttccaca
      601 acattacatt aggtagcaga ttggcagaga accaaagggt ggggccagta aggaaggact
      661 tctacacagt aaggaaacgc tgaaattcaa cttaatcact cagtcattta aaaataaata
      721 cttttatttt ttttattttt aaaggtctaa tggtttcatt agataagact gttattcctt
      781 gactgggatc atttagaacc atttgaagcc acatattgca ttttggaatt ttaaactaac
      841 aagctccaca atatagagaa aaaaatcttg aaactttttc aaaaacaatt tcttcattaa
      901 tgatgaaaaa aagggatcat ggatgaaaaa aggctaagta aattttgatt tgggaa
//
