LOCUS       sequence-158170-         956 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-myc3-CUL4B
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             72..101
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             108..137
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctat ggagcaaaag ctcatctcag aggaagatct ggaattagag caaaagctca
      121 tctcagagga agatctggaa ttagagcaaa agctcatctc agaggaagat ctggaattca
      181 ggatccctat gtcacagtca tctggatcag gagatgggaa tgatgatgag gctactacct
      241 ctaaagacgg tggtttttct tcccccagtc cctcagctgc tgctgctgct caggaggtca
      301 gatctgccac tgatggtaat accagcacca ctccgcccac ctctgccaag aagagaaagt
      361 taaacagcag cagcagtagc agcagtaaca gtagtaacga gagagaagac tttgattcca
      421 cctcttcctc ctcttccact cctcctttac aacccaggga ttcggcatcc ccttcaacct
      481 cgtccttctg cctgggggtt tcagtggctg cttccagcca cgtaccgata cagaagaagc
      541 tgcgttttga agacaccctg gagtttgtag ggtttgatgc gaagatggct gaggaatcct
      601 cctcctcctc ctcctcatct tcaccaactg ctgcaacatc tcagcagcag caacttaaaa
      661 ataagagtat attaatctct tctgtggctt cggtgcatca tgcaaacggc ctagccaaat
      721 cttctaccac cgtctctagc tttgctaaca gcaaacctgg ctctgctaag aagttagtga
      781 tcaagaactt taaagataag cctaaattac cagaaaacta cacagatgaa acctggcaaa
      841 aactgaaaga agcagtggaa gctattcaga atagtacttc aattaagtac aatttagaag
      901 aactctacca ggctgtagaa aatctctgtt cttacaagat ttctgcaaac ttgtac
//
