LOCUS       sequence-158900-         872 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  cLac94; pALM94; IPTG inducible system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF
  TITLE     Synthetic biology toolbox for controlling gene expression in the 
            cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. 
            Epub 2014 Sep 25.
  PUBMED    25216157
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500260k";
            journalName: "ACS Synth Biol"; date: "2015-05-15- 15"; volume: "4"; 
            issue: "5"; pages: "595-603"
COMMENT     Sequence Label: pACSA_PcptOO_YFP_PMB2__LacIWF
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..55)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(68..97)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    247..266
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(825..843)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(826..843)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atcgaggctg ctgcctgcat atgacgacct tcgatatggc
       61 cgctgctgtg atgatgatga tgatgatgat gatgatggcc catggaatta atctcctact
      121 tgactttatg agttggtgtg tgaaattgtt atccgctcac aattccacac atttatacaa
      181 cggtgatggg acttatgtct gttacatctt gttaatttta ttcctgcttt tttgttaaga
      241 attccgaatt gtgagcgctc acaattcgtt tgttcgtttc aatgaaggcg aaacgcccct
      301 gttgacccga ttcatccatt tcaatttggg ccacaaaaaa ttccttctgg acaatttccc
      361 cttcctctgt gaaggaaatt tcacccaaag gcgtgacgta ggtacctgca aaaatttcgt
      421 cccgcagttg tcgtcgcaag tccggtagag caagagtttc taagggcgtt ttttcatcga
      481 ggctgctgag ggcttcaaca aaaacttgga tcgccgtaaa agcctgggca ctaaattggg
      541 gcggctcttt ttggttttgt tgaaaatagg cgtcccgaaa cgcgcggtta atctcattat
      601 ctaactcggc actgtaggct tgggccacca acaccccatc acattttgct tggcagacgg
      661 ggaaaatatt agaagtattt aagccgttcc ccccaacaat aatgccgttg taacctaatt
      721 ctcgcagttg cttcaccagg ttgcccgcat cggctgctaa ccccgaaata atcactagat
      781 ctggttgaac attgagtgcg gcagtcacct catagcttga gtattctata gtgtcaccta
      841 aatagcttgg cgtaatcatg gtcatagctg tt
//
