LOCUS       sequence-159201-         770 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop 
            codon (for native or N-terminal fusions), Y40F mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4b Y40F
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             127..693
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSFRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(692..716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcaccatga ctgaatataa acttgtggta gttggagctg gtggcgtagg caagagtgcc
      181 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      241 tccttcagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      301 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      361 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      421 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      481 gatttgcctt ctagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      541 attcctttta ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca
      601 ttagttcgag aaattcgaaa acataaagaa aagatgagca aagatggtaa aaagaagaaa
      661 aagaagtcaa agacaaagtg tgtaattatg taatcaactt tcttgtacaa agttggcatt
      721 ataggaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
//
