LOCUS       sequence-159275-        1043 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of MAPK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MAPK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca gagcccaaga agtacgcagt
      181 gaccgacgac taccagttgt ccaagcaggt gctgggcctg ggtgtgaacg gcaaagtgct
      241 ggagtgcttc catcggcgca ctggacagaa gtgtgccctg aagctcctgt atgacagccc
      301 caaggcccgg caggaggtag accatcactg gcaggcttct ggcggccccc atattgtctg
      361 catcctggat gtgtatgaga acatgcacca tggcaagcgc tgtctcctca tcatcatgga
      421 atgcatggaa ggtggtgagt tgttcagcag gattcaggag cgtggcgacc aggctttcac
      481 tgagagagaa gctgcagaga taatgcggga tattggcact gccatccagt ttctgcacag
      541 ccataacatt gcccaccgag atgtcaagcc tgaaaaccta ctctacacat ctaaggagaa
      601 agacgcagtg cttaagctca ccgattttgg ctttgctaag gagaccaccc aaaatgccct
      661 gcagacaccc tgctatactc cctattatgt ggcccctgag gtcctgggtc cagagaagta
      721 tgacaagtca tgtgacatgt ggtccctggg tgtcatcatg tacatcctcc tttgtggctt
      781 cccacccttc tactccaaca cgggccaggc catctccccg gggatgaaga ggaggattcg
      841 cctgggccag tacggcttcc ccaatcctga gtggtcagaa gtctctgagg atgccaagca
      901 gctgatccgc ctcctgttga agacagaccc cacagagagg ctgaccatca ctcagttcat
      961 gaaccacccc tggatcaacc aatcgatggt agtgccacag accccactcc acacggcccg
     1021 agtgctgcag gaggacaaga acc
//
