LOCUS       sequence-159300-        1042 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of CSK21. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CSK21_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..129
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccggagagc tctttaatta agcggccgcc ctgcaggact cgagttctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg aaatcttctc accatcacca tcaccatcac
      121 catcaccatg aaaacctgta cttccaatcc aatgcaatgt cgggacccgt gccaagcaga
      181 gccagagttt acacagatgt taatacacac agacctcgag aatactggga ttacgagtca
      241 catgtggtgg aatggggaaa tcaagatgac taccagctgg ttcgaaaatt aggccgaggt
      301 aaatacagtg aagtatttga agccatcaac atcacaaata atgaaaaagt tgttgttaaa
      361 attctcaagc cagtaaaaaa gaagaaaatt aagcgtgaaa taaagatttt ggagaatttg
      421 agaggaggtc ccaacatcat cacactggca gacattgtaa aagaccctgt gtcacgaacc
      481 cccgccttgg tttttgaaca cgtaaacaac acagacttca agcaattgta ccagacgtta
      541 acagactatg atattcgatt ttacatgtat gagattctga aggccctgga ttattgtcac
      601 agcatgggaa ttatgcacag agatgtcaag ccccataatg tcatgattga tcatgagcac
      661 agaaagctac gactaataga ctggggtttg gctgagtttt atcatcctgg ccaagaatat
      721 aatgtccgag ttgcttcccg atacttcaaa ggtcctgagc tacttgtaga ctatcagatg
      781 tacgattata gtttggatat gtggagtttg ggttgtatgc tggcaagtat gatctttcgg
      841 aaggagccat ttttccatgg acatgacaat tatgatcagt tggtgaggat agccaaggtt
      901 ctggggacag aagatttata tgactatatt gacaaataca acattgaatt agatccacgt
      961 ttcaatgata tcttgggcag acactctcga aagcgatggg aacgctttgt ccacagtgaa
     1021 atcgccctgc agcctgggct tg
//
