LOCUS       sequence-159324-        8661 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Kukil K, Lindberg P
  TITLE     Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 
            6803 and subsequent improvements of sustainable production of 
            phenylpropanoids.
  JOURNAL   Microb Cell Fact. 2022 Jan 10;21(1):8. doi: 
            10.1186/s12934-021-01735-8.
  PUBMED    35012528
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-01-10"; volume: "21(1):8. doi"; pages: " 
            10.1186/s12934-021-01735-8"
COMMENT     Sequence Label: pEEK1
FEATURES             Location/Qualifiers
     source          1..8661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..28
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(63..68,69..85,86..92)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(179..484)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(281..300)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             832..855
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      881..952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      968..995
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1008..1065
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1089..1106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1247..1641
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1983..2070
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3309..4280
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4791..5630
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5617..6468
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6591..7406)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6591..7406)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7314..7333
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7398..7417
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8594..8612
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8616..8659)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattccgaa ttgtgagcgc tcacaattcg aacggttctg gcaaatattc tgaaatgagc
       61 tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc
      121 acacatactc gagtagtgga ggttctagag actggctgtg tataagggag cctgacattt
      181 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      241 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
      301 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
      361 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
      421 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      481 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
      541 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
      601 tcattctgca tggttgcgct taccagaccg gagatattga catcatatat gccttgagca
      661 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt
      721 tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa
      781 atacgcatac tgttatctgg cttttagtaa gccagatctg gtagcggaag ttggagtcat
      841 cctcagttcg agaagtaaac tagttaccgc ggccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat actgcagtcc ggcaaaaaag
     1021 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     1081 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gctcactcaa aggcggtaat caattgagtt cttttaccct cagccgaaat gcctgccgtt
     1201 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
     1261 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
     1321 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
     1381 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
     1441 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
     1501 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
     1561 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
     1621 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
     1681 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
     1741 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
     1801 atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
     1861 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
     1921 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
     1981 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc
     2041 gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc
     2101 tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt
     2161 atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg
     2221 actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat
     2281 ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt
     2341 atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga
     2401 ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc
     2461 gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga
     2521 tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt
     2581 acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg
     2641 catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg
     2701 ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc
     2761 ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc
     2821 gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat
     2881 accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac
     2941 gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc
     3001 cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa
     3061 gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc
     3121 gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag
     3181 ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg
     3241 ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg
     3301 gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg
     3361 gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg
     3421 aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg
     3481 gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg
     3541 acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag
     3601 tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg
     3661 aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg
     3721 ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca
     3781 cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg
     3841 ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg
     3901 agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc
     3961 gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg
     4021 acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg
     4081 aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc
     4141 acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc
     4201 ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg
     4261 ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact
     4321 cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga
     4381 tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg
     4441 aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt
     4501 ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa
     4561 gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg
     4621 cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac
     4681 gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt
     4741 agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc
     4801 ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt
     4861 tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat
     4921 ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg
     4981 gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc
     5041 atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg
     5101 acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt
     5161 tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc
     5221 ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg
     5281 aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg
     5341 cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg
     5401 ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg
     5461 gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg
     5521 gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg
     5581 tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac
     5641 agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc
     5701 ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc
     5761 aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa
     5821 gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa
     5881 ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgatgcc
     5941 atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt
     6001 ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc
     6061 gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag
     6121 gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc
     6181 ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc
     6241 ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata
     6301 gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc
     6361 cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag
     6421 ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc
     6481 tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt
     6541 aataccatgg taccgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     6601 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     6661 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     6721 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     6781 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     6841 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     6901 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     6961 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     7021 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     7081 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     7141 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     7201 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     7261 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     7321 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa
     7381 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     7441 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     7501 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagctcgag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8581 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
     8641 tttcgctaag gatgatttct g
//
