LOCUS       sequence-159344-         505 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid encodes the kinase domain of PAK4. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PAK4_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             97..126
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             127..147
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
       61 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatcaccat
      121 caccatgaaa acctgtactt ccaatccaat gcatcaccac agcgggagcc acagcgagta
      181 tcccatgagc agttccgggc tgccctgcag ctggtggtgg acccaggcga cccccgctcc
      241 tacctggaca acttcatcaa gattggcgag ggctccacgg gcatcgtgtg catcgccacc
      301 gtgcgcagct cgggcaagct ggtggccgtc aagaagatgg acctgcgcaa gcagcagagg
      361 cgcgagctgc tcttcaacga ggtggtaatc atgagggact accagcacga gaatgtggtg
      421 gagatgtaca acagctacct ggtgggggac gagctctggg tggtcatgga gttcctggaa
      481 ggaggcgccc tcaccgacat cgtca
//
