LOCUS       sequence-159356-        1025 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  This plasmid encodes the kinase domain of AURKA. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: AURKA_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             688..699
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcaa agaggcagtg ggctttggaa
      181 gactttgaaa ttggtcgccc tctgggtaaa ggaaagtttg gtaatgttta tttggcaaga
      241 gaaaagcaaa gcaagtttat tctggctctt aaagtgttat ttaaagctca gctggagaaa
      301 gccggagtgg agcatcagct cagaagagaa gtagaaatac agtcccacct tcggcatcct
      361 aatattctta gactgtatgg ttatttccat gatgctacca gagtctacct aattctggaa
      421 tatgcaccac ttggaacagt ttatagagaa cttcagaaac tttcaaagtt tgatgagcag
      481 agaactgcta cttatataac agaattggca aatgccctgt cttactgtca ttcgaagaga
      541 gttattcata gagacattaa gccagagaac ttacttcttg gatcagctgg agagcttaaa
      601 attgcagatt ttgggtggtc agtacatgct ccatcttcca ggaggaccac tctctgtggc
      661 accctggact acctgccccc tgaaatgatt gaaggtcgga tgcatgatga gaaggtggat
      721 ctctggagcc ttggagttct ttgctatgaa tttttagttg ggaagcctcc ttttgaggca
      781 aacacatacc aagagaccta caaaagaata tcacgggttg aattcacatt ccctgacttt
      841 gtaacagagg gagccaggga cctcatttca agactgttga agcataatcc cagccagagg
      901 ccaatgctca gagaagtact tgaacacccc tggatcacag caaattcatc aaaaccatca
      961 taataacatt ggaagtggat aacggatccg cgatcgcggc gcgccacctg gtggccggcc
     1021 ggtac
//
