LOCUS       sequence-159425-        8389 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-GCN5
FEATURES             Location/Qualifiers
     source          1..8389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     685..709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(813..815,816..818,819..1529)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(858..879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1119..1138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1466..1487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1545..1569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4084..4108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4116..4157
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4197..4216)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4241..4289
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4491..4919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4578..4597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4788..4809
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4928..4948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4933..5262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5113..5248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5175..5194
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5310..5357
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5376..5774
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5932..6053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5969..5988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6023..6042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6102..6118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6102..6118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6115..6137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6126..6142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6150..6156,6157..6174,6175..6180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6195..6216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6333..6350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6504..7092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6584..6603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7263..8054,8055..8123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7886..7905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8124..8228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8303..8322)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa
     1561 aagcaggctt catggcggaa ccttcccagg ccccgacccc ggccccggct gcgcagcccc
     1621 ggccccttca gtccccagcc cctgccccaa ctccgactcc tgcacccagc ccggcttcag
     1681 ccccgattcc gactcccacc ccggcaccag cccctgcccc agctgcagcc ccagccggca
     1741 gcacagggac tggggggccc ggggtaggaa gtgggggggc cgggagcggg ggggatccgg
     1801 ctcgacctgg cctgagccag cagcagcgcg ccagtcagag gaaggcgcaa gtccgggggc
     1861 tgccgcgcgc caagaagctt gagaagctag gggtcttctc ggcttgcaag gccaatgaaa
     1921 cctgtaagtg taatggctgg aaaaacccca agccccccac tgcaccccgc atggatctgc
     1981 agcagccagc tgccaacctg agtgagctgt gccgcagttg tgagcacccc ttggctgacc
     2041 acgtatccca cttggagaat gtgtcagagg atgagataaa ccgactgctg gggatggtgg
     2101 tggatgtgga gaatctcttc atgtctgttc acaaggaaga ggacacagac accaagcagg
     2161 tctatttcta cctcttcaag ctactgcgga aatgcatcct gcagatgacc cggcctgtgg
     2221 tggaggggtc cctgggcagc cctccatttg agaaacctaa tattgagcag ggtgtgctga
     2281 actttgtgca gtacaagttt agtcacctgg ctccccggga gcggcagacg atgttcgagc
     2341 tctcaaagat gttcttgctc tgccttaact actggaagct tgagacacct gcccagtttc
     2401 ggcagaggtc tcaggctgag gacgtggcta cctacaaggt caattacacc agatggctct
     2461 gttactgcca cgtgccccag agctgtgata gcctcccccg ctacgaaacc actcatgtct
     2521 ttgggcgaag ccttctccgg tccattttca ccgttacccg ccggcagctg ctggaaaagt
     2581 tccgagtgga gaaggacaaa ttggtgcccg agaagaggac cctcatcctc actcacttcc
     2641 ccaaattcct gtccatgctg gaggaggaga tctatggggc aaactctcca atctgggagt
     2701 caggcttcac catgccaccc tcagagggga cacagctggt tccccggcca gcttcagtca
     2761 gtgcagcggt tgttcccagc acccccatct tcagccccag catgggtggg ggcagcaaca
     2821 gctccctgag tctggattct gcaggggccg agcctatgcc aggcgagaag aggacgctcc
     2881 cagagaacct gaccctggag gatgccaagc ggctccgtgt gatgggtgac atccccatgg
     2941 agctggtcaa tgaggtcatg ctgaccatca ctgaccctgc tgccatgctg gggcctgaga
     3001 cgagcctgct ttcggccaat gcggcccggg atgagacagc ccgcctggag gagcgccgcg
     3061 gcatcatcga gttccatgtc atcggcaact cactgacgcc caaggccaac cggcgggtgt
     3121 tgctgtggct cgtggggctg cagaatgtct tttcccacca gctgccgcgc atgcctaagg
     3181 agtatatcgc ccgcctcgtc tttgacccga agcacaagac tctggccttg atcaaggatg
     3241 ggcgggtcat cggtggcatc tgcttccgca tgtttcccac ccagggcttc acggagattg
     3301 tcttctgtgc tgtcacctcg aatgagcagg tcaagggtta tgggacccac ctgatgaacc
     3361 acctgaagga gtatcacatc aagcacaaca ttctctactt cctcacctac gccgacgagt
     3421 acgccatcgg ctacttcaaa aagcagggtt tctccaagga catcaaggtg cccaagagcc
     3481 gctacctggg ctacatcaag gactacgagg gagcgacgct gatggagtgt gagctgaatc
     3541 cccgcatccc ctacacggag ctgtcccaca tcatcaagaa gcagaaagag atcatcaaga
     3601 agctgattga gcgcaaacag gcccagatcc gcaaggtcta cccggggctc agctgcttca
     3661 aggagggcgt gaggcagatc cctgtggaga gcgttcctgg cattcgagag acaggctgga
     3721 agccattggg gaaggagaag gggaaggagc tgaaggaccc cgaccagctc tacacaaccc
     3781 tcaaaaacct gctggcccaa atcaagtctc accccagtgc ctggcccttc atggagcctg
     3841 tgaagaagtc ggaggcccct gactactacg aggtcatccg cttccccatt gacctgaaga
     3901 ccatgactga gcggctgcga agccgctact acgtgacccg gaagctcttt gtggccgacc
     3961 tgcagcgggt catcgccaac tgtcgcgagt acaacccccc ggacagcgag tactgccgct
     4021 gtgccagcgc cctggagaag ttcttctact tcaagctcaa ggagggaggc ctcattgaca
     4081 aggacccagc tttcttgtac aaagtggttg ataacgggaa gcctatccct aaccctctcc
     4141 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
     4201 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     4261 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4321 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4381 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4441 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
     4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4921 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4981 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5041 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5161 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5221 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5281 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     5341 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     5401 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     5461 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     5521 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     5581 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     5641 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     5701 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     5761 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     5821 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5881 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5941 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6001 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6061 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6241 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6901 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8221 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     8281 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8341 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaat
//
