LOCUS       sequence-159433-        6425 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Kang X, Qi Y, Zuo Y, Wang Q, Zou Y, Schwartz RJ, Cheng J, Yeh ET
  TITLE     SUMO-specific protease 2 is essential for suppression of polycomb 
            group protein-mediated gene silencing during embryonic development.
  JOURNAL   Mol Cell. 2010 Apr 23. 38(2):191-201.
  PUBMED    20417598
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Apr 23. 38(2):191-201."
COMMENT     Sequence Label: FLAG-SENP2
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2766..3388
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2965..2984)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3412..3432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3417..3746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3597..3732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3784..3803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3785..3803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3817..3833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3817..3833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3830..3852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3841..3857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3865..3871,3872..3889,3890..3895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3910..3931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4048..4065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4219..4807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4299..4318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4978..5769,5770..5838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5601..5620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5839..5943)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5970..6398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6057..6076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6267..6288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattccatgt acagatggct ggttaggatt ctcggcacca ttttccgttt
     1021 ctgcgaccgg tcggtgcccc ctgcccgggc cctcctgaag aggcggcgct cagacagcac
     1081 tctgttttct acagtggaca ctgatgaaat accagccaaa agaccaagat tagattgctt
     1141 tattcaccaa gtgaaaaaca gtctctacaa tgctgccagc ttatttggat tcccattcca
     1201 gctgaccaca aagcccatgg taacttctgc ttgtaatgga acacggaatg tggccccttc
     1261 aggagaggta ttttcgaact cttcatcttg tgaactgaca ggttctggat cctggaacaa
     1321 catgctgaaa ctgggtaata aatctcctaa tggaataagt gactatccaa agatcagagt
     1381 gacagttacc cgagatcagc cacgcagagt cctgccttcc tttggtttta ctttgaactc
     1441 agaaggctgt aatagaagac caggtggccg tcgccatagc aaaggtaatc cagagagttc
     1501 tttaatgtgg aaacctcagg aacaggctgt aacagagatg atttctgaag agagtggcaa
     1561 gggtctgagg cgtccccatt gtactgtgga ggagggtgtt caaaaagagg aaagagagaa
     1621 gtaccgaaag ttattggaac gacttaaaga aagtggtcat ggaaactctg tctgtcctgt
     1681 aacttcaaat tatcacagtt ctcaaagaag tcagatggac acactaaaga ccaaaggctg
     1741 gggggaagag caaaatcacg gagtcaaaac aactcagttt gttccaaaac aatatagact
     1801 tgttgaaaca aggggacctc tatgttcatt gagaagtgaa aagaggtgtt caaaggggaa
     1861 aattactgat acagagacga tggtcggaat cagatttgaa aatgaaagta ggaggggata
     1921 ccaactggag cctgacctat cagaagaagt gtcggcccga ctccgcctgg gcagtggaag
     1981 caatggctta ctcaggagga aagtgtcaat aattgagaca aaggaaaaga attgctcagg
     2041 caaagagagg gacagaagaa cggacgatct ccttgaactt acagaggaca tggaaaagga
     2101 aatcagtaat gccctaggcc atggcccaca ggatgaaatc ctaagtagtg ctttcaaatt
     2161 gcgaattact cgaggagata ttcagacatt aaagaactat cactggctca atgatgaagt
     2221 cattaatttt tacatgaatc ttctggtgga aagaaataaa aagcaaggct atccagcact
     2281 tcatgtattc agtactttct tctatcctaa attaaagtct gggggttacc aagcagtgaa
     2341 acgatggacc aaaggggtaa atctctttga acaagaaatt attctggtgc ctattcatcg
     2401 gaaggtacat tggagcctgg tggtgattga cctaagaaaa aagtgtctta aatatctgga
     2461 ttctatggga caaaagggcc acaggatctg tgagattctc cttcagtatt tacaggatga
     2521 aagtaagacc aaaagaaata gtgatctgaa tcttttagag tggacccatc acagcatgaa
     2581 accacacgag attcctcaac agctgaatgg gagtgattgt ggaatgtttg cttgtaaata
     2641 tgcagattat atttctaggg acaaacctat cacatttact cagcaccaga tgcctctctt
     2701 ccggaagaag atggtgtggg aaatccttca tcagcagttg ctgtgagtcg actctagagg
     2761 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     2821 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     2881 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     2941 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     3001 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     3061 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     3121 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     3181 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     3241 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
     3301 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
     3361 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
     3421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3661 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3721 tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta
     3781 attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3961 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4021 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4621 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5941 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     6001 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6061 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6121 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6181 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6241 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6301 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6361 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6421 aattt
//
