LOCUS       sequence-159548-         982 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-27a-3p in three copies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR-27a-3p 3 perfect copies.
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..125
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(225..976)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
       61 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      121 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gggcccttct
      181 taatgttctt agggctttac caacagtacc ggaatgccaa gcttttaagc gggtcgctgc
      241 agggtcgctc ggtgttcgag gccacacgcg tcaccttaat atgcgaagtg gacctgggac
      301 cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa tgacaagacg ctgggcgggg
      361 tttgtgtcat catagaacta aagacatgca aatatatttc ttccggggac accgccagca
      421 aacgcgagca acgggccacg gggatgaagc agctgcgcca ctccctgaag ctcctgcagt
      481 ccctcgcgcc tccgggtgac aagatagtgt acctgtgccc cgtcctggtg tttgtcgccc
      541 aacggacgct ccgcgtcagc cgcgtgaccc ggctcgtccc gcagaaggtc tccggtaata
      601 tcaccgcagt cgtgcggatg ctccagagcc tgtccacgta tacggtcccc atggagccta
      661 ggacccagcg agcccgtcgc cgccgcggcg gcgccgcccg ggggtctgcg agcagaccga
      721 aaaggtcaca ctctggggcg cgcgacccgc ccgagtcagc ggcccgccag ttaccacccg
      781 ccgaccaaac ccccgcctcc acggagggcg ggggggtgct taagaggatc gcggcgctct
      841 tctgcgtgcc cgtggccacc aagaccaaac cccgagccgc ctccgaatga gagtgtttcg
      901 ttccttcccc ctccccccgc gtcagacaaa ccctaaccac cgcttaagcg gcccccgcga
      961 ggtccgaaga ctcatttaga tc
//
