LOCUS       sequence-159556-         704 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-19b-3p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR 19b-3p
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     102..123
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(223..368)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
       61 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      121 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg gcccttctta
      181 atgttcttag ggctttacca acagtaccgg aatgccaagc ttttaagcgg gtcgctgcag
      241 ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctgggaccg
      301 cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt
      361 tgtgtcatca tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa
      421 cgcgagcaac gggccacggg gatgaagcag ctgcgccact ccctgaagct cctgcagtcc
      481 ctcgcgcctc cgggtgacaa gatagtgtac ctgtgccccg tcctggtgtt tgtcgcccaa
      541 cggacgctcc gcgtcagccg cgtgacccgg ctcgtcccgc agaaggtctc cggtaatatc
      601 accgcagtcg tgcggatgct ccagagcctg tccacgtata cggtccccat ggagcctagg
      661 acccagcgag cccgtcgccg ccgcggcggc gccgcccggg ggtc
//
