LOCUS       sequence-159562-         938 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor Control insert. Three 
            Perfect copies of complementary region of a siRNA control targeting 
            firefly luciferase encoded by the pGL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0-GL-2-3 perfect copies
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(130..843,844..846,847..849))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(175..196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     524..543
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     783..804
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(857..875)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(918..937)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 atgtaatgaa aataaagata ttttattgcg gccagcggcc cacgtacgcg gaatacttcg
       61 aaaccggcac gtacgcggaa tacttcgaaa ccggcacgta cgcggaatac ttcgaaatcg
      121 agcgatcgct tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      181 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      241 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      301 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagtgggc
      361 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctga tgtagttgta
      421 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      481 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      541 gtccttgaag aagatggtac gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      601 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc aggtcagggt
      661 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      721 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaacc tgtggccgtt
      781 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      841 gctcaccatt ggctagccta tagtgagtcg tattaagtac tctagcctta agagctgtaa
      901 ttgaactggg agtggacacc tgtggagaga aaggcaaa
//
