LOCUS       sequence-159573-         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of redox sensitive GFP with a mitochondrial targeting 
            sequence in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pEGFP-N1-mt-ro2GFP
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      278..287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(284..286,287..289,290..802)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(329..350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(590..609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcgaattcg ggctgaagga
       61 gacttggggg cacccgcgtc gtgcctcctg ggttgtgagg agtcgccgct gccgccactg
      121 cctgtgcttc atgaggaaga tgctcgccgc cgtctcccgc gtgctgtctg gcgcttctca
      181 gaagccggca agcagagtgc tggtagcatc ccgtaatttt gcaaatgatg ctacatttct
      241 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      301 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      361 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      421 gttcatctcc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      481 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      541 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      601 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      661 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      721 caactgccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      781 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact ac
//
