LOCUS       sequence-159583-         889 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro4GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..787)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHCVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLCTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(114..134)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     729..757
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(806..820,821..829))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(833..865)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(869..886)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctttgttag cagccggatc aagcttcgaa ttccatggta ccagctgcag atctcgagct
       61 cggatcctta ttatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa
      121 actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg
      181 tgcacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt
      241 gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa
      301 ctttgattcc attcttttgt ttgtctgcca tgatgtatac acagtgtgag ttatagttgt
      361 attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga
      421 ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt
      481 catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa
      541 agtcatgccg tttcatatga tctgggtatc ttgaaaagca ttgaacacca taagtgaaag
      601 tagtgacaag tgttggccat ggaacaggta gttttccagt agtggaaata aatttaaggg
      661 taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat
      721 taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt
      781 tactcatgaa ttcagcgggg ggatccttat cgtcatcgtc gtacagatcc cgacccattt
      841 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgaga
//
