LOCUS       sequence-160110-         870 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a high-affinity ePDZ variant fused to mCherry in yeast. 
            Illumination with 440 nm light induces binding to LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS221
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    119..171
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(807..823)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
       61 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg cgaccggtgg
      121 atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga gtctagaggt
      181 acggtagtta atcgagattg ggctagagta gtaatggtag ttgtaatgtg cgtatacagt
      241 gatggtatag tcgacacccg gtttcaggcc gctgatggta gcagtagact tggaaccagg
      301 tacagtgaat tcctgaaccg gggagttacc accggtttca ccgtacgtga tacggtaata
      361 actaaccgga agctccctat acgcatccca gctgatcagc aggctagtcg gggtcgcagc
      421 aacaacttcc agattggtcg gaacagaaga aacgcctcca gaaccaccgt ctttttcaac
      481 cctcactcga atctcttgtt ttgcaccgtt accaacttct cgtacaatga tgagttcaac
      541 tgtattctgg aaagttttta gcaaggacac tgcttgtcca tgttcaatat ttataaaact
      601 gtagccatta gcctgaataa ttttatcacc tggctgcagt aattttgatg ctggtccttc
      661 aggttgtacc cttgttacaa atataccatc atcatcaggt ctgaatgggt ttcctctacc
      721 cccgacacca cctgatatgc taaatccaag ttctggcatt gtcgacggta tcgataagct
      781 tgatatcgaa ttcctgcagc ccgggggatc cactagttct agaaaactta gattagattg
      841 ctatgctttc tttctaatga gcaagaagta
//
