LOCUS       sequence-160193-         912 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  To introduce MS2 coat protein fused Tet1-CD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu GL, Hu 
            R
  TITLE     A CRISPR-based approach for targeted DNA demethylation.
  JOURNAL   Cell Discov. 2016 May 3;2:16009. doi: 10.1038/celldisc.2016.9. 
            eCollection 2016.
  PUBMED    27462456
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-05-3"; volume: "2:16009. doi"; pages: " 
            10.1038/celldisc.2016.9. eCollection 2016"
COMMENT     Sequence Label: pcDNA3.1-MS2-Tet1-CD
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..503
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             117..503
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             555..575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accatggctt
      121 ctaactttac tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc
      181 caagcaactt cgctaacggg gtcgctgaat ggatcagctc taactcgcgt tcacaggctt
      241 acaaagtaac ctgtagcgtt cgtcagagct ctgcgcagaa gcgcaaatac accatcaaag
      301 tcgaggtgcc taaagtggca acccagactg ttggtggtgt agagcttcct gtagccgcat
      361 ggcgttcgta cttaaatatg gaactaacca ttccaatttt cgccacgaat tccgactgcg
      421 agcttattgt taaggcaatg caaggtctcc taaaagatgg aaacccgatt ccctcggcca
      481 tcgcagcaaa ctccggcatc tacagcgctg gaggaggtgg aagcggagga ggaggaagcg
      541 gaggaggagg tagccccaag aagaagagaa aggtggaggc cagcggtgga ggaagtggag
      601 gtggttcagg tggaggatcc ggcgaagctg caccctgtga ctgtgatgga ggtacacaaa
      661 aagaaaaagg cccatattat acacaccttg gggcaggacc aagtgtggct gctgtcaggg
      721 agctcatgga gactaggttt ggccagaagg ggaaggcaat ccggattgag aagatagtgt
      781 tcacggggaa ggaagggaag agctctcagg gctgcccggt cgccaagtgg gtgatcagaa
      841 gaagtggtcc tgaagagaag cttatttgtt tggttcgtga gcgtgtagac catcactgtt
      901 cgacggctgt ga
//
