LOCUS       sequence-160360-        1481 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bioluminescent Reporter of Intracellular Calcium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson
            CH
  TITLE     Coupling optogenetic stimulation with NanoLuc-based luminescence 
            (BRET) Ca++ sensing.
  JOURNAL   Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268.
  PUBMED    27786307
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-27"; volume: "7:13268. doi"; pages: " 
            10.1038/ncomms13268"
COMMENT     Sequence Label: pT7-CalfluxVTN
FEATURES             Location/Qualifiers
     source          1..1481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(58..79)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(319..338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     666..687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             934..1449
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgcccgca tgcaagatgc tagcgaagaa
      721 gagctatcag agtgctttcg catttttgac aaggatggaa atggtttcat cgacagggag
      781 gaatttggag acatcatccg ccttactgga gaacagctca cagatgagga tcccgatgag
      841 atatttggag attcagacac agacaaaaat ggaaggattg actttgatga gttcctgaag
      901 atggtggaga atgtccagcc tctggccgag ctcatggtct tcacactcga agatttcgtt
      961 ggggactggc gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg
     1021 tccagtttgt ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc
     1081 ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc
     1141 gaccaaatgg gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac
     1201 tttaaggtga tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc
     1261 gactatttcg gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta
     1321 acagggaccc tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc
     1381 tccctgctgt tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt
     1441 ctggcgtaag gtaccaagct taaggcggcc gcactcntca g
//
