LOCUS       sequence-160571-         913 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  fluorescent Isce joint substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Neal JA, Xu Y, Abe M, Hendrickson E, Meek K
  TITLE     Restoration of ATM Expression in DNA-PKcs-Deficient Cells Inhibits 
            Signal End Joining.
  JOURNAL   J Immunol. 2016 Apr 1;196(7):3032-42. doi: 10.4049/jimmunol.1501654.
            Epub 2016 Feb 26.
  PUBMED    26921311
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501654"; journalName: "J Immunol"; date: 
            "2016-04-1- 1"; volume: "196"; issue: "7"; pages: "3032-42"
COMMENT     Sequence Label: isce rfp/cfp
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..785
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGALKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(238..256)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(288..308)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     506..525
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     700..723
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      842..851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagccca agcttgggct gcaggtcgaa
       61 gttacgctag ggataacagg gtaatatagt cgactctcat cgataccatg gcctcctccg
      121 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      181 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      241 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
      301 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc
      361 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      421 tgaccgtgac ccaggactcc tccctgcagg acggaagctt catctacaag gtgaagttca
      481 tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg
      541 cctccaccga gcgcctgtac ccccgcgacg gcgcgctgaa gggcgagatc cacaaggccc
      601 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga
      661 agcccgtgca gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca
      721 acgaggacta caccatcgtg gagcagtacg agcgcgccga gggccgccac cacctgttcc
      781 tgtagcatcg atgagaggat cctatattac cctgttatcc ctagcgtaac tgataccggt
      841 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      901 gctggacggc gac
//
