LOCUS       sequence-160614-        1054 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gateway Donor vector containing PNKP , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PNKP_WT
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagttggca ccatgggcga ggtggaggcc ccgggccgct
      121 tgtggctcga gagcccccct gggggagcgc cccccatctt cctgccctcg gacgggcaag
      181 ccctggtcct gggcagggga cccctgaccc aggttacgga ccggaagtgc tccagaactc
      241 aagtggagct ggtcgcagat cctgagaccc ggacagtggc agtgaaacag ctgggagtta
      301 acccctcaac taccgggacc caggagttga agccggggtt ggagggctct ctgggggtgg
      361 gggacacact gtatttggtc aatggcctcc acccactgac cctgcgctgg gaagagaccc
      421 gcacaccaga atcccagcca gatactccgc ctggcacccc tctggtgtcc caagatgaga
      481 agagagatgc tgagctgccg aagaagcgta tgcggaagtc aaaccccggc tgggagaact
      541 tggagaagtt gctagtgttc accgcagctg gggtgaaacc ccagggcaag gtggctggct
      601 ttgatctgga cgggacgctc atcaccacac gctctgggaa ggtctttccc actggcccca
      661 gtgactggag gatcttgtac ccagagattc cccgtaagct ccgagagctg gaagccgagg
      721 gctacaagct ggtgatcttc accaaccaga tgagcatcgg gcgcgggaag ctgccagccg
      781 aggagttcaa ggccaaggtg gaggctgtgg tggagaagct gggggtcccc ttccaggtgc
      841 tggtggccac gcacgcaggc ttgtaccgga agccggtgac gggcatgtgg gaccatctgc
      901 aggagcaggc caacgacggc acgcccatat ccatcgggga cagcatcttt gtgggagacg
      961 cagccggacg cccggccaac tgggccccgg ggcggaagaa gaaagacttc tcctgcgccg
     1021 atcgcctgtt tgccctcaac cttggcctgc cctt
//
