LOCUS       sequence-160817-        9612 bp    DNA     circular SYN 14-APR-2025
DEFINITION  stable overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLJM5-WT PHGDH
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2023..2140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2632..2651
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     LTR             4120..4353
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4425..4546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4462..4481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4516..4535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4586..4721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4741..4760)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4742..4760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4770..4787)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4770..4786)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4779..4801)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4928..5383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5015..5034)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5225..5246
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5409..5513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5514..5582,5583..6374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5732..5751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6545..7133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7034..7053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7287..7304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7421..7442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7457..7462,7463..7480,7481..7487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7495..7511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7500..7522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7519..7535
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7519..7535
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7554..7574
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7556..7574
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7602..7828
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7829..8009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8056..8181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8674..8907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9092..9136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9434..9454
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcccagcaat ggcttttgca aatctgcgga aagtgctcat cagtgacagc ctggaccctt
      421 gctgccggaa gatcttgcaa gatggagggc tgcaggtggt ggaaaagcag aaccttagca
      481 aagaggagct gatagcggag ctgcaggact gtgaaggcct tattgttcgc tctgccacca
      541 aggtgaccgc tgatgtcatc aacgcagctg agaaactcca ggtggtgggc agggctggca
      601 caggtgtgga caatgtggat ctggaggccg caacaaggaa gggcatcttg gttatgaaca
      661 cccccaatgg gaacagcctc agtgccgcag aactcacttg tggaatgatc atgtgcctgg
      721 ccaggcagat tccccaggcg acggcttcga tgaaggacgg caaatgggag cggaagaagt
      781 tcatgggaac agagctgaat ggaaagaccc tgggaattct tggcctgggc aggattggga
      841 gagaggtagc tacccggatg cagtcctttg ggatgaagac tatagggtat gaccccatca
      901 tttccccaga ggtctcggcc tcctttggtg ttcagcagct gcccctggag gagatctggc
      961 ctctctgtga tttcatcact gtgcacactc ctctcctgcc ctccacgaca ggcttgctga
     1021 atgacaacac ctttgcccag tgcaagaagg gggtgcgtgt ggtgaactgt gcccgtggag
     1081 ggatcgtgga cgaaggcgcc ctgctccggg ccctgcagtc tggccagtgt gccggggctg
     1141 cactggacgt gtttacggaa gagccgccac gggaccgggc cttggtggac catgagaatg
     1201 tcatcagctg tccccacctg ggtgccagca ccaaggaggc tcagagccgc tgtggggagg
     1261 aaattgctgt tcagttcgtg gacatggtga aggggaaatc tctcacgggg gttgtgaatg
     1321 cccaggccct taccagtgcc ttctctccac acaccaagcc ttggattggt ctggcagaag
     1381 ctctggggac actgatgcga gcctgggctg ggtcccccaa agggaccatc caggtgataa
     1441 cacagggaac atccctgaag aatgctggga actgcctaag ccccgcagtc attgtcggcc
     1501 tcctgaaaga ggcttccaag caggcggatg tgaacttggt gaacgctaag ctgctggtga
     1561 aagaggctgg cctcaatgtc accacctccc acagccctgc tgcaccaggg gagcaaggct
     1621 tcggggaatg cctcctggcc gtggccctgg caggcgcccc ttaccaggct gtgggcttgg
     1681 tccaaggcac tacacctgta ctgcaggggc tcaatggagc tgtcttcagg ccagaagtgc
     1741 ctctccgcag ggacctgccc ctgctcctat tccggactca gacctctgac cctgcaatgc
     1801 tgcctaccat gattggcctc ctggcagagg caggcgtgcg gctgctgtcc taccagactt
     1861 cactggtgtc agatggggag acctggcacg tcatgggcat ctcctccttg ctgcccagcc
     1921 tggaagcgtg gaagcagcat gtgactgaag ccttccagtt ccacttctaa tgaggtacca
     1981 tgaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2041 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2101 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2161 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
     2221 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     2281 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     2341 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     2401 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt ctcgtgcaga
     2461 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     2521 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     2581 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     2641 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     2701 cctgcatccc gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     2761 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     2821 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     2881 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     2941 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     3001 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     3061 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     3121 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     3181 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     3241 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     3301 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     3361 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     3421 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     3481 gcttgcagga tcgccgcggc tccggggcgt atatgctccg cattggtctt gaccaactct
     3541 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     3601 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     3661 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     3721 ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa caagagctga tttcgagaac
     3781 gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg gccttacgct
     3841 tggtggcaca gttctcgtcc acagttcgct aagctcgctc ggctgggtcg cgggagggcc
     3901 ggtcgcagtg attcaggccc ttctggattg tgttggtccc cagggcacga ttgtcatgcc
     3961 cacgcactcg ggtgatctga ctgatcccgc agattggaga tcgccgcccg tgcctgccga
     4021 ttgggtgcag atccgtcgac ctgcagccaa gctctttaag accaatgact tacaaggcag
     4081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4141 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4321 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     4381 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4561 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4741 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4801 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4921 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4981 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5041 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5101 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5161 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5221 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5281 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5341 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5401 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5461 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5521 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5581 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5641 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5701 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5761 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5821 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5881 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5941 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6001 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6061 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6121 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6181 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6241 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6301 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6361 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6421 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7201 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7261 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7381 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7441 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7501 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7561 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7621 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7681 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7741 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7801 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7981 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8041 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     8101 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     8161 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     8221 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     8281 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     8341 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     8401 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     8461 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8521 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8581 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8641 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8701 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8761 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8821 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8881 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8941 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9001 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9061 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9121 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9181 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9241 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9301 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     9361 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
     9421 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     9481 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     9541 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     9601 aatggactat ca
//
